Research Specialist / Scientist, Metabolic Phenotyping (In Vitro & In Vivo)
About the role
Our Mission Our mission is to restore cell health and resilience through cell rejuvenation to reverse disease, injury, and the disabilities that can occur throughout life. For more information, see our website at altoslabs.com. Our Value Our Single Altos Value: Everyone Owns Achieving Our Inspiring Mission . Diversity at Altos Altos Labs has been named one of the Top 3 Biotech Companies and ranked for the second year on the Forbes 2026 Best Startups in America list. At Altos, exceptional scientists and industry leaders from around the world work together to advance a shared mission. Our intentional focus is on Belonging, so that all employees know that they are valued for their unique perspectives. We are all accountable for sustaining a diverse and inclusive environment. What You Will Contribute To Altos You will join a discovery team investigating
the role of metabolism in aging and cellular rejuvenation. You will independently plan, execute, troubleshoot, and interpret rigorous experiments across models that range from primary cell cultures to in vivo rodent systems, applying a diverse set of techniques that includes real-time measurements of cellular energetics, multi-omics sample generation, metabolic imaging, and whole-animal phenotyping. You will formulate hypotheses and take ownership of your experimental projects, drawing on guidance from senior colleagues to work through roadblocks. You will communicate findings clearly to both scientific and non-scientific colleagues, collaborate across
the team, and keep the meticulous, well-organized electronic-laboratory-notebook records that make
the team's data rigorous and reproducible. Key
Responsibilities Design and execute in vivo rodent metabolic studies in collaboration with
the team, including animal handling, dosing (intraperitoneal, intravenous, oral), metabolic phenotyping (glucose- and insulin-tolerance tests, CLAMS for metabolic monitoring), and endpoint tissue collection optimized for multi-omics, bioenergetic, and histological analysis. Perform immunofluorescence and immunohistochemistry on cultured cells and metabolic tissues (e.g., liver, adipose, muscle), and operate fluorescence, confocal, or automated high-content imaging systems to visualize lipid droplets, mitochondrial morphology, and protein localization. Design and execute metabolic flux assays on the Agilent Seahorse XF analyzer, and prepare cellular and tissue extracts for downstream mass spectrometry-based metabolomics and lipidomics and for genomics workflows (RNA/DNA isolation, quantitative PCR). Analyze and interpret bioenergetic, imaging, and phenotyping data (Wave, ImageJ/FIJI, GraphPad Prism), drawing accurate conclusions that guide the next round of experiments. Maintain primary metabolic cell cultures and develop, optimize, and scale the throughput of cell-based assays. Document all work with accurate, timely records in the electronic laboratory notebook (ELN), and coordinate sample tracking and submissions with internal teams and external contract research organizations (CROs). Communicate results clearly through written summaries and presentations, and contribute to manuscripts and conference presentations. Collaborate across the discovery team to align in vitro mechanism-of-action findings with in vivo efficacy readouts.
